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Aunt May (May Parker)

Played by Marisa Tomei, Rosemary Harris, Sally Field, Lily Tomlin.

Marvel Cinematic Universe

• Spider-Man: Homecoming (2017), played by Marisa Tomei
• Spider-Man: Far From Home (2019), played by Marisa Tomei
• Spider-Man: No Way Home (2021), played by Marisa Tomei
• Spider-Man: Brand New Day (2026), played by Marisa Tomei

Spider-Man

• Spider-Man (2002), played by Rosemary Harris
• Spider-Man 2 (2004), played by Rosemary Harris
• Spider-Man 3 (2007), played by Rosemary Harris
• The Amazing Spider-Man (2012), played by Sally Field
• The Amazing Spider-Man 2 (2014), played by Sally Field
• Spider-Man: Into the Spider-Verse (2018), played by Lily Tomlin